Plant Transcription Factor Database
Previous version: v3.0
Dichanthelium oligosanthes
HSF Family
Species TF ID Description
Do001166.1HSF family protein
Do001347.1HSF family protein
Do001799.1HSF family protein
Do001981.1HSF family protein
Do002645.1HSF family protein
Do003167.1HSF family protein
Do005082.1HSF family protein
Do005859.1HSF family protein
Do006714.1HSF family protein
Do007201.1HSF family protein
Do007631.1HSF family protein
Do008079.1HSF family protein
Do012237.1HSF family protein
Do013762.1HSF family protein
Do016287.1HSF family protein
Do017593.1HSF family protein
Do017926.1HSF family protein
Do018433.1HSF family protein
Do019511.1HSF family protein
Do021403.1HSF family protein
Do021609.1HSF family protein
Do021675.1HSF family protein
Do023137.1HSF family protein
Do025927.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645